Released on: September 17, 2008, 1:54 pm

Press Release Author: AnaSpec Inc.

Industry: Biotech

Press Release Summary: AnaSpec announced today that BD Biosciences, a business
segment of BD (Becton Dickinson and Company), has chosen it to manufacture and
supply the BD™ H7, Calcein AM and DiIC12(3) Fluorescent Dyes.

Press Release Body: September 17, 2008 – San Jose, CA

AnaSpec announced today that BD Biosciences, a business segment of BD (Becton
Dickinson and Company), has chosen it to manufacture and supply the BD™ H7, Calcein
AM and DiIC12(3) Fluorescent Dyes.

“BD Biosciences has a tremendous reputation in the industry for innovative products
of the highest quality,” stated Dr. Anita Hong, AnaSpec’s president. “We are proud
to leverage AnaSpec’s fluorescent dye technology in the production of these novel
dyes.”

H7 is the abbreviation of HiLyte FluorTM 750 Bis-NHS ester, which carries two
amine-reactive groups, succinimidyl esters, on each dye molecule for general
labeling of protein amine groups.

BD APC-H7 is a tandem conjugate and an analog of APC-CyTM7 with the same spectral
properties. It has decreased intensity but it is engineered for greater stability
and less spillover in the APC channel and consequently offers better performance
than APC-Cy7. It has an absorption maximum of approximately 650 nm. When excited
by light from a red laser, the APC fluorochrome can transfer energy to the cyanine
dye, which then emits at a longer wavelength. The resulting fluorescent emission
maximum is approximately 767 nm.

The cell viability indicator calcein AM, is a non-fluorescent, cell permeant
compound that is hydrolyzed by intracellular esterases into the fluorescent anion
calcein. BD™ Calcein AM Fluorescent Dyes can be used to fluorescently pre- and
post-label viable cells to perform kinetic and endpoint experiments, respectively.
DiIC12(3) is a lipophilic neuronal tracer, which is commonly used for the labeling
of neuronal projections as well as lipid bilayers in other cell types. Since
DiIC12(3) exhibits low toxicity and minimal effects on cell viability, this dye can
be used to pre-label cells for a variety of applications.

BD™ DiIC12(3) Fluorescent Dye can be used to fluorescently label viable cells for
assays, such as tumor cell invasion or endothelial cell migration. Moreover, cells
are known to tolerate different fluorescent dyes for varying amounts of time in
culture. While cells labeled with calcein AM should be exposed to the dye for less
than eight hours, DiIC12(3) can be used for several days in culture without
adversely affecting cells.
About AnaSpec
AnaSpec, Inc. is a leading provider of integrated proteomics solutions™ for
worldwide life science research. With a vision for innovation through synergy,
AnaSpec offers expertise in three primary technologies: peptides, detection
reagents, and combinatorial chemistry. Founded in 1993 and headquartered in San
Jose, California, AnaSpec serves the world’s largest pharmaceutical, biotech, and
academic research institutions. For more information visit www.anaspec.com
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Web Site: http://www.anaspec.com

Contact Details: AnaSpec Inc.
2149 O'Toole Ave.
San Jose, CA 95131
1-408-452-5055 (Tel)
1-408-452-5059 (Fax)
1-800-452-5530
ping@anaspec.com
www.anaspec.com